POSTER PRESENTATION ABSTRACTS
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Salons 10-12.
THURSDAY, 10:15-11:00 AM
Tsuiji K, Toda T, Kurihara N, Imazu Y, Ishige A, Yamamoto
M, Watanabe K.
Influence of blood ammonia and intestinal flora in partially
hepatectomized mice with Juzen-Taiho-To.
Kampo medicine, Keio University school of Medicine
di055036@sc.itc.keio.ac.jp
PURPOSE: In this study, we investigated the
blood ammonia level and intestinal flora in partially hepatectomized
mice. We also observed the effect of JTT on the blood ammonia change
and intestinal flora.
METHOD: 7-week-old male BALB/c mice were partially
hepatectomized and then divided into a JTT (1.0g/kg) group and a placebo
group (water). Unoperated mice with no medication were treated as control
group. The medicine was orally administered once a day from one week
before the partial hepatectomy through three days after the operation.
Blood ammonia was checked by ammonia analyzer (DRI-CHEM 100; FUJIFILM).
Feces were collected for three days after the operation and the intestinal
flora was analyzed using Terminal-Restriction Fragment Length Polymorphism
(T-RFLP) (MspI). DNA was extracted from the feces by QIAamp DNA Stool
Mini Kit (QIAGEN).
RESULTS: In this study, blood ammonia level
was measured to investigate effects of the medicine on hepatectomized
animals. The increased blood ammonia levels after the operation were
significantly lowered in JTT 0.5g/kg group and JTT 1.0g/kg group compared
to the group to which no JTT was administered. A significant change
in pattern of intestinal flora was observed in operated groups compared
to the unoperated group. The change in pattern of intestinal bacteria
was insignificant in groups with JTT.
CONCLUSIONS: Blood ammonia was elevated three
days after hepatectomy. JTT suppressed elevated blood ammonia caused
by hepatectomy. Pattern of intestinal flora was changed due to hepatectomy.
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