POSTER PRESENTATION ABSTRACTS
Please note: All posters will be displayed in
Salons 10-12.
FRIDAY, 5:45-6:30 PM
Tsai SY, Chi YC, Chao PDL.
A single-point method for detecting
pharmacokinetic interaction of Chinese herbs with Neoral® in rats.
School of Pharmacy, China Medical University sytsai@mail.cmu.edu.tw
Cyclosporine is an immunosuppressive agent with narrow
therapeutic window. Neoral® is a microemulsion formulation of cyclosporine.
This study aimed to establish an economic and efficient method for detecting
pharmacokinetic interaction of Chinese herbs with Neoral® in rats.
Sprague-Dawley rats were given Neoral® orally and blood samples
were withdrawn via cardiopuncture at 0, 0.33, 0.66, 1, 3, 5, 7 and 9
h post dosing of Neoral®. Fluorescence polarization immunoassay
was used to determine the blood cyclosporin concentration. The area
under the serum concentration - time curve (AUC0-t) was calculated by
the trapezoidal rule to the last point using noncompartment model of
WINNONLIN. Linear regressions were made between the blood cyclosporine
levels at various time points of each rat with correspondent AUCs and
the correlation coefficients were compared. Our results indicated that
the cyclosporin blood levels at I h and 3 h post dosing showed high
correlation with AUCs. The correlation coefficients were 0.77 and 0.83,
respectively. Employing single-point sampling at 1 h or 3 h post dosing
of Neoral®, the pharmacokinetic interaction of Chinese herbs with
Neoral® can be efficiently detected.
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