POSTER PRESENTATION ABSTRACTS
Please note: All posters will be displayed in
Salons 10-12.
THURSDAY, 5:30-6:15 PM
Rai S, Wahile A, Mukherjee K, Saha B P, Mukherjee PK.
Antioxidant activity of Nelumbo nucifera (sacred lotus)
seeds.
School of Natural Product Studies, Department
of Pharmaceutical Technology, Jadavpur University sujayrai@gmail.com,
rai_sujay@yahoo.com
Antioxidant activity of hydro alcoholic extract of
Nelumbo nucifera seeds (HANN) was studied using in vitro
and in vivo models. Total phenolic content in HANN was found
to be 7.61 ± 0.04 % w/w. Characteristic HPTLC fingerprints of HANN were
also made using different solvent systems. The HANN exhibited strong
free radical scavenging activity as evidenced by the low IC50 values
in both DPPH (1, 1-diphenyl-2-picryl hydrazyl) (6.12 ± 0.41_g/m1) and
nitric oxide (84.86 ± 3.56_g/m1) methods. The values were found to be
less than those of rutin, the standard used. Acute toxicity of HANN
was evaluated in Swiss Albino mice, no signs of toxicity were observed
up to the oral dose of 1000 mg/kg body weight. Administration of HANN
to Wistar rats at 100 and 200 mg kg/body weight for four days prior
to carbon tetrachloride (CCl4) treatment caused a significant dose dependent
increase (p<0.05 to p<0.001) in the level of superoxide dismutase (SOD)
and Catalase and a significant decrease (p<0.05 to p<0.001) in the level
of thiobarbituric acid reactive substances (TBARS), when compared to
CCl4 treated control in both liver and kidney. These changes observed
at 100 mg/kg body weight treatment were comparable to those observed
for standard vitamin E at 50 mg/kg treatment. N. nucifera seeds
contain alkaloids, saponins, phenolics and carbohydrates. The results
support significant antioxidant nature of HANN.
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