POSTER PRESENTATION ABSTRACTS
Please note: All posters will be displayed in
Salons 10-12.
FRIDAY, 10:15-11:00 AM
Ling YC, Pei-Hsun H, Hsiu SL, Hou YC*.
Pharmacokinetics of a Chinese herbal formula San-Huang-Shel-Shin-Tang
in rats.
School of Chinese Medicine, China Medical University
houyuchi@yahoo.com.tw
*Corresponding
author
San-Huang-Shel-Shin-Tang (SHSST) is a popular traditional
Chinese medicine formula, including Da Huang (Rhei Rhizoma), Huang Lien
(Coptidis Rhizoma) and Huang Qin (Scutellariae Radix). Till now, traditional
decoction is still a dosage form used in clinical Chinese medicine.
However, there is no report concerning the pharmacokinetics of SHSST.
In order to provide the data base for clinical application, this study
attempted to assess the bioavailability and pharmacokinetics of polyphenols
and alkaloids in SHSST decoction.
The quantitation method of coptisine, palmatine, berberine, baicalin,
baicalein, wogonin, emodin, aloe-emodin, rhein, chrysophanol using HPLC
was developed and validated in this study. The aglycones and glycosides
of each constituent were determined prior to and after hydrolysis with
glucosidase. After oral administration of SHSST decoction, rat blood
samples were withdrawn via cardiopuncture at specific time points. The
aglycones and conjugates metabolites were measured by HPLC prior to
and after hydrolysis with sulfatase and β-glucuronidase, respectively.
The pharmacokinetic parameters were calculated using noncompartment
model of WINNONLIN. The results showed that the aglycones increased
considerably after hydrolysis, indicating that polyphenols of SHSST
exist mainly as glycosides. After oral administration of SHSST decoction,
coptisine, palmatine and berberine were not detected in serum, flavonoids
and anthraquinones were found predominantly as sulfates and glucuronides,
only free form of rhein was detected in the bloodstream.
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