POSTER PRESENTATION ABSTRACTS
Please note: All posters will be displayed in
Salons 10-12.
THURSDAY, 5:30-6:15 PM
Lin B, Saul I, Busto R, Fernandez G, Guillerm L.
Combination of 9 herbal medicines protects neurons in
hippocampal CA1 after global ischemia in rat brain.
C V D Research Center, University of Miami, FL
lin@stroke.med.miami.edu
In the present study, we employed 9 Chinese
herbal medicines, focusing on antioxidant and anti-inflammation which
showed beneficial in ischemia patients, to conduct research in rat brain
and investigate the mechanism. The major components penetrate blood
brain barrier quickly and evenly distribute in intact and ischemic brain
in 20 minutes after oral administration. Male Wistar rats, aged 2.3-3.1
months and weighted 256-340g, were subjected to 12.5-min global forebrain
ischemia induced by common carotid arteries occlusion plus systemic
hypotension (50 mm Hg). Head temperature was controlled at 36-36.5oC
and body temperature was at 37-37.5oC by warming lamps over the head
and body. Plasma glucose, pO2 and pCO2 were in normal ranges. The dried
medical plants were boiled in water to make cocktail-type soup, i.e.
decoction. Rats orally received the 9-herb decoction (treated group,
n=7) or water (control, n=5) through feed needle for 3 days initiated
at 4 hours after insult. Rats survived for 3 days. Sham rats (n=4) received
surgery but not ischemia. Brains were perfusion-fixed with FAM (formaldehyde,
acetic acid, and methanol; 1:1:8 in volume). Sections were stained by
hematoxylin and eosin. Normal-appearing pyramidal neurons in hippocampus
CA1 sector at the level ~3.3 mm posterior to bregma were counted under
microscope. All of the medial, middle, and lateral sub-sectors in CA1
were evidently necrotic in every rat. The numbers of normal-appearing
neurons in CA1 were 96 ± 68 in treated group and 24 ± 18 in control
groups at survival of 3 days (mean ± S.D.; ANOVA t-test, p=0.03)
while 438 ± 73 in sham rats.
CONCLUSION: The results indicate the multiple-targeting
combination of 9 herbal medicines protected the neurons in hippocampal
CA1 from global ischemia in rat brain.
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