POSTER PRESENTATION ABSTRACTS
Please note: All posters will be displayed in Salons 10-12.

THURSDAY, 10:15-11:00 AM


Lin B, Saul I, Busto R, Fernandez G, Guillerm L.

Combination of Chinese herbal medicine alleviated acute intracerebral hemorrhage (ICH) injury in rats.

C V D Research Center, University of Miami, FL lin@stroke.med.miami.edu

PURPOSE: ICH has been much less studied. Strategies, we consider, include removing hematoma and decreasing damage in perihematoma regions.

METHODS: We employed 2 hemorrhage models, consisting of injecting either 100 µl of blood into striatum or 50 µl into cortex in 12 rats (n=3 in each group including treated and control ones). Rats were orally administrated an herb for 2 days to arrest bleeding and resolve or dispel extravasated blood and our 9-herb decoction for 6 days to attenuate the peri-hematoma edema and remove the blood and its degradations, initiated at 2 hrs after blood injection. Beam-walking test was performed after ICH. Brains were collected on the 7th day survival for histological exam. Since the first herb contains ginsenoside Rb1, it is able to stabilize the membrane structure of mitochondria of neurons. In cortex protocol, treated rats received a one-time intravenous injection of a compound from another natural medicine at 1 hr after hemorrhage to enhance the mitochondrial protection.

RESULTS: Histological exam revealed that hematoma disappeared in treated rats while it persisted in nontreated ones in the cortex protocol. The peri-hematoma structural destruction was less severe in treated rats than control group, and beam-walking test showed that treated rats traverse at least 2 days earlier in the striatal protocol. The pilot studies imply these 11 natural medicines encouraged CNS healing and functional recovery after brain hemorrhage.

CONCLUSION: The multiple targeting and combination therapy made of by the 11 natural medicines displayed the neuroprotection in ICH damage in rats.

 

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